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Fluorescence laser tracking microrheology for quantitative studies of cytoskeletal mechanotransduction Alternative Title: FLTM for quantitative studies of cytoskeletal mechanotransduction Author: Jonas, Maxine Other Contributors: Massachusetts Institute of Technology.
Massachusetts Institute of Technology Date Issued: To shed light on the cell's response to its mechanical environment, we examined cell rheology at the single cell level and quantified it with nanometer spatial and microsecond temporal resolutions over a five-decade frequency range - 0.
To this end, we developed and optimized an instrument for fast fluorescence laser tracking microrheology FLTM. This novel method aims at experimentally deriving cellular viscoelastic properties from the passive monitoring of fluorescent microspheres undergoing Brownian motion inside the tested sample.
Further instrument enhancement even broadens the FLTM frequency span up to seven decades by modulating data acquisition speed or complementing FLTM with a two-particle microrheology modality.
In living cells, FLTM accurately characterizes the solid-like vs. FLTM also demonstrates the existence of two distinct rheological regimes on the cell surface and in the cell interior: Finally, FLTM evaluates frequency-specific stretch-induced cell mechanics and thus promises to broaden and diversify the scientific knowledge on mechanotransduction, from a molecular and cellular standpoint.by Yohane Kachale (Malawi) on 24/Oct/ Verified Buyer.
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ground based laser system located at Kirtland AFB, New Mexico. Much thanks goes to my thesis advisor, Dr.
Maybeck, for his patience, consideration, and above all . Hearst Television participates in various affiliate marketing programs, which means we may get paid commissions on purchases made through our links to retailer sites.
One case was found that used a Leica AT laser tracker, but it also measured deflections by measuring the elevation angle, i.e., like the total station cases, the measurement architecture did not exploit the higher accuracy and speed of EDM. A laser tracker from Leica Geosystems is a portable measurem ent system that relies on a absolute interferometer to accurately measure and inspec t in a radial volume of up to [ m ].
A long-term standard in industrial metrology: Laser Tracker Systems lead the field in terms of the accuracy, reliability and durability of portable coordinate measuring machines.